megaTALs: a rare-cleaving nuclease architecture for therapeutic genome engineering.

Boissel S, Jarjour J, Astrakhan A, Adey A, Gouble A, Duchateau P, Shendure J, Stoddard BL, Certo MT, Baker D, Scharenberg AM
Nucleic Acids Res. 2013 Nov 26. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
50420	pCVL TAL(Nd154C63) SFFV Xba-Sal linker	pTAL plasmid compatible with the Golden Gate TALEN cloning system. For cloning of megaTALs or other TAL-fusions and expression in mammalian cells. Meganuclease/fusion is cloned between Xba-Sal sites.
50421	pCVL TAL(Nd154C63) SFFV Age-Xho/Xba-Sal linkers	pTAL plasmid for cloning of megaTALs or other TAL-fusions and expression in mammalian cells. RVDs are cloned between Age-Xho sites and meganuclease/fusion is cloned between Xba-Sal sites.
50422	pFUSC2	Plasmid compatible with the Golden Gate TALEN cloning system. To clone TAL effectors 2.5 RVDs long.
50423	pFUSC3	Plasmid compatible with the Golden Gate TALEN cloning system. To clone TAL effectors 3.5 RVDs long.
50424	pFUSC4	Plasmid compatible with the Golden Gate TALEN cloning system. To clone TAL effectors 4.5 RVDs long.
50425	pFUSC5	Plasmid compatible with the Golden Gate TALEN cloning system. To clone TAL effectors 5.5 RVDs long.
50426	pFUSC6	Plasmid compatible with the Golden Gate TALEN cloning system. To clone TAL effectors 6.5 RVDs long.
50427	pFUSC7	Plasmid compatible with the Golden Gate TALEN cloning system. To clone TAL effectors 7.5 RVDs long.
50428	pFUSC8	Plasmid compatible with the Golden Gate TALEN cloning system. To clone TAL effectors 8.5 RVDs long.
50429	pFUSC9	Plasmid compatible with the Golden Gate TALEN cloning system. To clone TAL effectors 9.5 RVDs long.
50430	pFUSC10	Plasmid compatible with the Golden Gate TALEN cloning system. To clone TAL effectors 10.5 RVDs long.
50434	pCVL SFFV Sbf-Sal linker	Cloning vector for coexpression of gene cloned in between Sbf and Sal sites with BFP in mammalian cells. Made for cloning of meganuclease for direct comparison with megaTALs (pCVL TAL Xba-Sal).