Autoregulation of Connexin43 Gap Junction Formation by Internally Translated Isoforms.

Smyth JW, Shaw RM
Cell Rep. 2013 Nov 5. pii: S2211-1247(13)00578-0. doi: 10.1016/j.celrep.2013.10.009. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
49851	pcDNA3.2-Cx43-HA	Encodes human connexin 43 with a C-terminal HA epitope fusion tag
49852	pcDNA3.2-Cx43-siResist	Encodes human connexin 43 with wobble mutations conferring resistance against an siRNA
49853	pcDNA3.2-Cx43-NoStart	Encodes human connexin 43 with the start methionine codon mutated to isoleucine and with wobble mutations conferring resistance against an siRNA
49854	pcDNA3.2-Cx43-M100L-siResist	Encodes human connexin 43 with M100 mutated to L and wobble mutations conferring resistance against an siRNA
49855	pcDNA3.2-Cx43-M100/125L-siResist	Encodes human connexin 43 with M100 and M125 mutated to L and wobble mutations conferring resistance against an siRNA
49856	pcDNA3.2-Cx43-M100/125/147L-siResist	Encodes human connexin 43 with M100, M125, and M147 mutated to L and wobble mutations conferring resistance against an siRNA
49857	pcDNA3.2-Cx43-ML-HA	Encodes human connexin 43 with all internal methionines mutated to leucine and a C_terminal HA epitope fusion tag
49858	pcDNA3.2-Cx43-M100/125/147/213-siResist	Encodes human connexin 43 with M100, M125, M147, and M213 mutated to L and wobble mutations conferring resistance against an siRNA
49859	pcDNA3.2-GJA1-20K-V5	Encodes C-terminal fragment of human connexin 43 initiating at M213, with M281 and M320 mutated to L, and a V5 C-terminal fusion tag
49860	pDEST-EGFP-Cx43ML-N1	Encodes human connexin 43 with all internal methionines mutated to leucine and a C_terminal EGFP fusion tag
49861	pDEST-mCherry-GJA1-20k-N1	Encodes C-terminal fragment of human connexin 43 initiating at M213 with a V5 C-terminal fusion tag