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Modular optimization of multi-gene pathways for fatty acids production in E. coli.

Xu P, Gu Q, Wang W, Wong L, Bower AG, Collins CH, Koffas MA
Nat Commun. 2013;4:1409. doi: 10.1038/ncomms2425. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
49808pCDM4-GLYEncodes pgk, gapA, aceE, aceF, lpdA in pseudo-operon configuration.
49809pACM4-ACAEncodes fabD, accA, accB, accC, accD in pseudo-operon configuration.
49810pETM6-FASEncodes CnfatB2, fabA, fabH, fabG, fabI in pseudo-operon configuration
66865pETM6-M.accABCDEncodes accA, accB, accC, accD in monocistronic configuration.
66866pETM6-P.accABCDEncodes accA, accB, accC, accD in Pseudo-operon configuration.

Antibodies from Article