New vectors for epitope tagging and gene disruption in Schizosaccharomyces pombe.
Gadaleta MC, Iwasaki O, Noguchi C, Noma K, Noguchi E
Biotechniques. 2013 Nov;55(5):257-63. doi: 10.2144/000114100.
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Article
Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
33138 | pFA6a-LEU2MX6 | gene deletion in S. cerevisiae and S. pombe |
33139 | pFA6a-his3MX6 | gene deletion in S. pombe |
33140 | pFA6a-GFP-ura4MX6 | genomic GFP tagging in yeast |
33141 | pFA6a-GFP-bleMX6 | genomic GFP tagging in yeast |
49176 | pFA6a-12Pk-kanMX6 | genomic 12Pk epitope tagging in S. pombe |
49177 | pFA6a-12Pk-hphMX6 | genomic 12Pk epitope tagging in S. pombe |
49179 | pFA6a-12Pk-LEU2MX6 | genomic 12Pk epitope tagging in S. pombe |
49180 | pFA6a-12Pk-ura4MX6 | genomic 12Pk epitope tagging in S. pombe |
49181 | pFA6a-G9-5FLAG-kanMX6 | genomic G9-5FLAG epitope tagging in S. pombe |
49182 | pFA6a-G11-5FLAG-kanMX6 | genomic G11-5FLAG epitope tagging in S. pombe |
49183 | pFA6a-mRFP-ura4MX6 | genomic mRFP tagging in S. pombe |
49184 | pFA6a-ura4MX6 | gene deletion in S. pombe |
49185 | pFA6a-G9-5FLAG-hphMX6 | genomic G9-5FLAG epitope tagging in S. pombe |