A genetic strategy to identify targets for the development of drugs that prevent bacterial persistence.
Kim JH, O'Brien KM, Sharma R, Boshoff HI, Rehren G, Chakraborty S, Wallach JB, Monteleone M, Wilson DJ, Aldrich CC, Barry CE 3rd, Rhee KY, Ehrt S, Schnappinger D
Proc Natl Acad Sci U S A. 2013 Nov 4.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 49517 | pGMCK-0X750-luxAB-DAS+4 | Kanamycin resistant; expresses luxAB-DAS+4 under the control of a promoter containing PtetO-4C5G; integrates into the mycobacteriophage att-L5 site |
| 49518 | pGMCK-T38S38-750-luxAB-DAS+4 | Kanamycin resistant; expresses luxAB-DAS+4 under the control of a promoter containing PtetO-4C5G; constitutively expresses reverse TetR (tetR38); integrates into the mycobacteriophage att-L5 site |
| 49519 | pGMEH-0X750-luxAB-DAS+4 | Hygromycin resistant; expresses luxAB-DAS+4 under the control of a promoter containing PtetO-4C5G; replicates episomally |
| 49520 | pGMEH-T38M-750-luxAB-DAS+4 | Hygromycin resistant; expresses luxAB-DAS+4 under the control of a promoter containing PtetO-4C5G; constitutively expresses reverse TetR (tetR38); replicates episomally |
| 49521 | pGMCtN-TSC10M1-sspB | Nourseothricin resistant; expresses sspB under the control of a promoter containing PtetO and constitutively expressses single-chain TetR10 (tsc10); integrates into mycobacteriophage att-Tweety site |
| 49522 | pGMCKq20-T38S38-0X | Kanamycin resistant; contains qtag20 and constitutively expresses reverse TetR (tetR38); integrates into the mycobacteriophage att-L5 site |
| 49523 | pDE43-MCK | Kanamycin and Chloramphenicol resistant; destination vector engineered to integrate into the mycobacteriophage att-L5 site and designed to be used with the multisite Gateway cloning system |
| 49524 | pEN41A-T38S38 | Ampicillin resistant; entry clone encoding reverse TetR (tetR38) and contains attL4 and attL1 sites for recombination into a Gateway destination vector at the attR4 and attR1 sites |
| 49525 | pEN12A-P750 | Ampicillin resistant; entry clone containing PtetO-4C5G and attL1 and attL2 sites for recombination into a Gateway destination vector at the attR4 and attR1 sites |
| 49526 | pGMCtKq28-TSC10M1-sspB | Kanamycin resistant; contains qtag28, expresses sspB under a promoter containing PtetO, and constutively expresses single-chain TetR10 (tsc10); integrates into the mycobacteriophage att-Tweety site |