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Controlling gene expression in yeast by inducible site-specific recombination.

Cheng TH, Chang CR, Joy P, Yablok S, Gartenberg MR
Nucleic Acids Res. 2000 Dec 15;28(24):E108. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
49455pSH62-EBDyeast expression of Cre recombinase fused to the estrogen binding domain (EBD), which confers estradiol-dependent control to Cre recombinase
49456pRKO loxP-kanMX-loxP cassette inserted into the ACT1 intron for PCR amplification of the intron/kanMX module for one-step homology-driven genomic disruptions

Antibodies from Article