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A nanobody-based system using fluorescent proteins as scaffolds for cell-specific gene manipulation.

Tang JC, Szikra T, Kozorovitskiy Y, Teixiera M, Sabatini BL, Roska B, Cepko CL
Cell. 2013 Aug 15;154(4):928-39. doi: 10.1016/j.cell.2013.07.021. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
49438pCAG-GBP1-10gly-Gal4DBDExpression of a GFP-dependent transcription factor component in mammalian cells
49439pCAG-Gal4DBD-GBP2Expression of a GFP-dependent transcription factor component in mammalian cells
49440pCAG-p65AD-GBP6Expression of a GFP-dependent transcription factor component in mammalian cells
49441pCAG-GBP7-p65ADExpression of a GFP-dependent transcription factor component in mammalian cells
50020pCS2+ Gal4-GBP2-IRES-GBP7-p65Multi-purpose expression of a GFP-dependent transcription factor
50444pCAG-GFPmG1Expression of a EGFP variant in mammalian cells
50791pCAG-rTetRDBD-GBP1Expression of a GFP-dependent transcription factor component in mammalian cells
50795pCAG-GBP1-10gly-lexADBDExpression of a GFP-dependent transcription factor component in mammalian cells
50796pCAG-GBP6-10gly-VPminx4Expression of a GFP-dependent transcription factor component in mammalian cells
50797pUAS-CreExpression of Cre recombinase under the control of the UAS-promoter
50798pTRE-tdTomatoExpression of tdTomato under the control of the TRE-promoter

Antibodies from Article