Simon Bullock Lab: CRISPRflydesign
Unpublished
CRISPR fly design is a genome engineering project in Drosophila, consisting of transgenic cas9 fly lines and gRNA plasmids. Project tools can be used for both generating knockouts and precise modifications using HDR.
For more information about this project, please refer to the CRISPR Fly Design (Link opens in a new window) homepage.
gRNA plasmids
| ID | Name | Description | PI |
|---|---|---|---|
| 49408 | pCFD1-dU6:1gRNA | expression of gRNA under control of the Drosophila U6:1 promoter | Bullock |
| 49409 | pCFD2-dU6:2gRNA | expression of gRNA under control of the Drosophila U6:2 promoter | Bullock |
| 49410 | pCFD3-dU6:3gRNA | expression of gRNA under control of the Drosophila U6:3 promoter | Bullock |
| 49411 | pCFD4-U6:1_U6:3tandemgRNAs | expression of two gRNAs from Drosophila U6:1 and U6:3 | Bullock |
| 73914 | pCFD5 | multiplex gRNA plasmid | Bullock |
| 73915 | pCFD6 | tissue-specific multiplex gRNA plasmid | Bullock |
Cas9 plasmids
| ID | Name | Description | PI |
|---|---|---|---|
| 62209 | pAct-Cas9 | Expresses Cas9 under control of act5C promoter and SV40 3'UTR. For ubiquitous genome engineering in Drosophila melanogaster. | Bullock |
| 62208 | pnos-Cas9-nos | Expresses Cas9 under control of nanos promoter and 3'UTR. For germ line restricted genome engineering in Drosophila melanogaster. | Bullock |
| 62211 | pAct-Fok1:dCas9 | Expresses Fok1:dCas9 under control of act5C promoter and SV40 3'UTR. For ubiquitous high specificity genome engineering in Drosophila melanogaster. | Bullock |
| 62210 | pnos-Fok1:dCas9-nos | Expresses Fok1:dCas9 under control of nanos promoter and 3'UTR. For germ line restricted high specificity genome engineering in Drosophila melanogaster. | Bullock |
Additional References
- Port, F., Chen, H., Lee, T., & Bullock, S. L. (2014). Optimized CRISPR/Cas tools for efficient germline and somatic genome engineering in Drosophila. Proceedings of the National Academy of Sciences, 111(29). https://doi.org/10.1073/pnas.1405500111.
- Port, F., & Bullock, S. L. (2016). Augmenting CRISPR applications in Drosophila with tRNA-flanked sgRNAs. Nature Methods, 13(10), 852–854. https://doi.org/10.1038/nmeth.3972.
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 62210 | pnos-Fok1:dCas9-nos | Expresses Fok1:dCas9 under control of nanos promoter and 3'UTR. For germ line restricted high specificity genome engineering in Drosophila melanogaster. |
| 62211 | pAct-Fok1:dCas9 | Expresses Fok1:dCas9 under control of act5C promoter and SV40 3'UTR. For ubiquitous high specificity genome engineering in Drosophila melanogaster. |