Genome engineering using the CRISPR-Cas9 system.
Ran FA, Hsu PD, Wright J, Agarwala V, Scott DA, Zhang F
Nat Protoc. 2013 Nov;8(11):2281-308. doi: 10.1038/nprot.2013.143. Epub 2013 Oct 24.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 48137 | pSpCas9 (PX165) | Human codon optimized Cas9 nuclease from Streptococcus pyogenes (Cbh-3X-FLAG-NLS-SpCas9-NLS) |
| 48138 | pSpCas9(BB)-2A-GFP (PX458) | Cas9 from S. pyogenes with 2A-EGFP, and cloning backbone for sgRNA |
| 48139 | pSpCas9(BB)-2A-Puro (PX459) | NOTE: A new version of this plasmid is now available. See Addgene plasmid 62988. |
| 48140 | pSpCas9n(BB)-2A-GFP (PX461) | Cas9n (D10A nickase mutant) from S. pyogenes with 2A-EGFP, and cloning backbone for sgRNA |
| 48141 | pSpCas9n(BB)-2A-Puro (PX462) | NOTE: A new version of this plasmid is now available. See Addgene plasmid 62987 |
| 48873 | pSpCas9n(BB) (PX460) | Cas9n (D10A nickase mutant) from S. pyogenes |
| 62987 | pSpCas9n(BB)-2A-Puro (PX462) V2.0 | Cas9n (D10A nickase mutant) from S. pyogenes with 2A-Puro, and cloning backbone for sgRNA (V2.0) |
| 62988 | pSpCas9(BB)-2A-Puro (PX459) V2.0 | Cas9 from S. pyogenes with 2A-Puro, and cloning backbone for sgRNA (V2.0) |