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Characterization of cytoplasmic caspase-2 activation by induced proximity.

Bouchier-Hayes L, Oberst A, McStay GP, Connell S, Tait SW, Dillon CP, Flanagan JM, Beere HM, Green DR
Mol Cell. 2009 Sep 24;35(6):830-40. doi: 10.1016/j.molcel.2009.07.023. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
49257pBiFC-HA-hCaspase 2 FL (C303S)-VC155Expresses split fluorescence protein to measure caspase-2 dimerization in mammalian cells.
49258pBiFC-flag-hCaspase 2 FL (C303S)-VN173Expresses split fluorescence protein to measure caspase-2 dimerization in mammalian cells.
49259pBiFC-HA-hCaspase 2 CARD-VC155Expresses split fluorescence protein to measure caspase-2 dimerization in mammalian cells.
49260pBiFC-flag-hCaspase 2 CARD-VN173Expresses split fluorescence protein to measure caspase-2 dimerization in mammalian cells.
49261pBiFC-HA-hCaspase 2 prodomain-VC155Expresses split fluorescence protein to measure caspase-2 dimerization in mammalian cells.
49262pBIFC-flag-hCaspase 2 prodomain-VN173Expresses split fluorescence protein to measure caspase-2 dimerization in mammalian cells.

Antibodies from Article