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Genetically encoded calcium indicator illuminates calcium dynamics in primary cilia.

Su S, Phua SC, Derose R, Chiba S, Narita K, Kalugin PN, Katada T, Kontani K, Takeda S, Inoue T
Nat Methods. 2013 Sep 22. doi: 10.1038/nmeth.2647. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
474995HT6-G-GECO1.0Fluorescent reporter for Ca2+ inside primary cilia
475005HT6-mCherry-G-GECO1.0Fluorescent reporter for Ca2+ inside primary cilia with mCherry reference
475015HT6-CFP-Venus(H148G)Fluorescent reporter for pH inside primary cilia
483395HT6-YC3.60FRET reporter for Ca2+ inside primary cilia
483405HT6-GCaMP5GFluorescent reporter for Ca2+ inside primary cilia
48342TNXXL-CTS20FRET reporter for Ca2+ inside primary cilia
48343TNXXL-CTS68FRET reporter for Ca2+ inside primary cilia

Antibodies from Article