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Single molecule imaging reveals a major role for diffusion in the exploration of ciliary space by signaling receptors.

Ye F, Breslow DK, Koslover EF, Spakowitz AJ, Nelson WJ, Nachury MV
Elife. 2013 Aug 6;2:e00654. doi: 10.7554/eLife.00654. Print 2013. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
49098pEF5B-FRT-AP-Sstr3-GFP-DESTExpressing AP-Sstr3-GFP in mammalian cells, can be used for establishing Flp-In stable cell lines
49099pEF5B-FRT-AP-Smo-YFP-DESTExpressing AP-Smo-YFP in mammalian cells, can be used for establishing Flp-In stable cell lines
61684pEF5-FRT-TagRFP-T-IFT88Mammalian expression of IFT88 fused with N terminal TagRFP.T tag

Antibodies from Article