Efficient site-specific transgenesis and enhancer activity tests in medaka using PhiC31 integrase.
Kirchmaier S, Hockendorf B, Moller EK, Bornhorst D, Spitz F, Wittbrodt J
Development. 2013 Sep 18.
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PubMed
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 48875 | pSB_PhiC31LandingSite | Sleeping beauty transposon with the PhiC31 landing site in a cmlc2::EGFP selection cassette |
| 48876 | pEx_MCS-attBtagRFPt | PhiC31 exchange vector with multiple cloning site, exchange of EGFP in landing site with tagRFPt |
| 48877 | pEx_Hsp70::EGFPpA-ttBtagRFPt | PhiC31 exchange vector with the Hsp70::EGFPpA insert. Selection via EGFP to tagRFPt-color switch in the heart |
| 48878 | pEx_Hsp70::attBmut | PhiC31 exchange vector with the Hsp70 promoter driving tagRFPt from the landing site. Template for enhancer tests. |