Transgene-Free Genome Editing in Caenorhabditis elegans Using CRISPR-Cas.

Chiu H, Schwartz HT, Antoshechkin I, Sternberg PW
Genetics. 2013 Aug 26. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

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ID	Plasmid	Purpose
47912	SP6-sgRNA-scaffold	Scaffold into which clone a 20 bp targeting sequence to generate a plasmid for in vitro transcription of an sgRNA using SP6 RNA polymerase for use in CRISPR-Cas by RNA injection
47911	SP6-hCas9-Ce-mRNA	In vitro transcription of a mRNA encoding humanized Cas9 nuclease, with 3' UTRs suitable for germline expression in C. elegans, for doing CRISPR-Cas by RNA injection. Uses SP6 RNA polymerase.