mTORC1 phosphorylation sites encode their sensitivity to starvation and rapamycin.
Kang SA, Pacold ME, Cervantes CL, Lim D, Lou HJ, Ottina K, Gray NS, Turk BE, Yaffe MB, Sabatini DM
Science. 2013 Jul 26;341(6144):1236566. doi: 10.1126/science.1236566.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 48799 | pLJM60 mUlk1 (wt) barcoded | expresses wt Ulk1 |
| 48800 | pLJM60-S6K1(T389S) barcoded | expresses mutant S6K1 |
| 48801 | pLJM60-S6K1(wt) barcoded | expresses wt S6K1 |
| 48805 | pRK5 HA-GST-Presc-mAkt1 (wt) | expresses wt Akt1 |
| 48806 | pRK5 HA-GST-Presc-mAkt1 (S473T) | expresses mutant Akt1 |