Competing E3 ubiquitin ligases govern circadian periodicity by degradation of CRY in nucleus and cytoplasm.
Yoo SH, Mohawk JA, Siepka SM, Shan Y, Huh SK, Hong HK, Kornblum I, Kumar V, Koike N, Xu M, Nussbaum J, Liu X, Chen Z, Chen ZJ, Green CB, Takahashi JS
Cell. 2013 Feb 28;152(5):1091-105. doi: 10.1016/j.cell.2013.01.055.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 48752 | pCMV10-Flag-Fbxl21 | Expression of FLAG-tagged Fbxl21 |
| 51888 | pCMV10-Flag-Ovtm | expression vectore in mammalian cells |
| 51890 | pTre2tet)::Fbxl21 | expression of Fbxl21 |
| 51892 | pfast bac cry1myc | Backbone plasmid is pfastBacHTa from invitrogen |
| 51893 | pfast bac Rbx-HA | Backbone plasmid is pfastBacHTa from invitrogen |
| 51894 | pfast bac-flag Fbxl3 | Backbone plasmid is pfastBacHTa from invitrogen |
| 110297 | mcry1K31R HA tag | mammalian expression of full length mouse CRY1 mutant K31R |
| 110298 | mCRY1 HA tag | full length wt mouse Cry1 HA tag in pcDNA3.1 |