Protein ligation in living cells using sortase.
Strijbis K, Spooner E, Ploegh HL
Traffic. 2012 Jun;13(6):780-9. doi: 10.1111/j.1600-0854.2012.01345.x. Epub 2012 Mar 23.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 50029 | pRS303-pGAL-HA-SrtAstrep (pKS78) | Galactose-inducible expression of cytosolic S. pyogenes SrtA in yeast |
| 50030 | pcDNA3.1(-)-HA-SrtAstrep (pKS77) | Expression of cytosolic S. pyogenes SrtA in mammalian cells |
| 50031 | pcDNA3.1(-)-PM-Cpalm-HA-SrtA (pKS37) | Expression of S. pyogenes SrtA at the plasma membrane in mammalian cells |
| 50032 | pRS303-pGAL-SPPHO5-G-SrtAstrep-HA-HDEL (pKS82) | Galactose-inducible expression of ER-luminal S. pyogenes SrtA in yeast |
| 50033 | pRS303-pGAL-HA-TMSec66-SrtA (pKS105) | Galactose-inducible expression of ER-membrane S. pyogenes SrtA in yeast |