Strategies to construct null and conditional null Trypanosoma brucei mutants using Cre-recombinase and loxP.
Kim HS, Li Z, Boothroyd C, Cross GA
Mol Biochem Parasitol. 2013 Aug 13;191(1):16-19. doi: 10.1016/j.molbiopara.2013.08.001.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 48355 | pSY45 | Targets TbMCM-BP for Cre-lox mediated knockout in T. brucei |
| 48356 | pHJ17 | Targets beta-Tubulin for Cre-lox mediated knockout in T. brucei, Hygromycin/Gancyclovir selectable |
| 48357 | pHJ18 | Targets beta-Tubulin for Cre-lox mediated knockout in T. brucei, Puromycin//Gancyclovir selectable |
| 48358 | pHJ22 | Targets beta-Tubulin for Cre-lox mediated knockout in T. brucei, Neomycin/Gancyclovir selectable |
| 48359 | pHJ42 | Targets beta-Tubulin for Cre-lox mediated knockout in T. brucei, Bleomycin/Gancyclovir selectable |
| 48360 | pHJ41 | Targets beta-Tubulin for Cre-lox mediated knockout in T. brucei, Blasticidin/Gancyclovir selectable |
| 48361 | pHJ32 | Targets beta-Tubulin for Cre-lox mediated knockout in T. brucei, Hygromycin selectable |
| 48362 | pHJ33 | Targets beta-Tubulin for Cre-lox mediated knockout in T. brucei, Puromycin selectable |
| 48364 | pDS66 | 3XHA-intergenic region of TUB-HYG (modified from PMID 16269191) |
| 48365 | pDS67 | 3XMYC-intergenic region of TUB-HYG (modified from PMID 16269191) |
| 48366 | pDS68 | GFP-intergenic region of TUB-HYG (modified from PMID 16269191) |
| 48367 | pDS69 | FLAG-intergenic region of TUB-HYG (modified from PMID 16269191) |