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Efficient generation of large-scale genome-modified mice using gRNA and CAS9 endonuclease.

Fujii W, Kawasaki K, Sugiura K, Naito K
Nucleic Acids Res. 2013 Aug 30. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
48625pCAG-T3-hCAS-pAExpresses CAS9 nuclease in mammalian cells. Used to modify genome of mouse embroys
59766pCAG-T3-hCASeGFP-pAExpresses CAS9-eGFP fusion protein in mammalian cells.

Antibodies from Article