A prodrug resistance mechanism is involved in colibactin biosynthesis and cytotoxicity.
Brotherton CA, Balskus EP
J Am Chem Soc. 2013 Mar 6;135(9):3359-62. doi: 10.1021/ja312154m. Epub 2013 Feb 20.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 48243 | pET29b-ClbPpep-CHis | First 375 amino acids of the peptidase, which contains the soluble periplasmic peptidase domain |
| 48244 | pET29b-ClbP | Untagged, full-length protein for in vivo peptidase assays |
| 48245 | pET29b-ClbP-S95A | Untagged, full-length protein with active site mutation Serine95 to Alanine for in vivo peptidase assays |
| 49217 | pet28a-ClbB-NRPS-NHis | NRPS module of ClbB containing CAT domains with N-terminal 6His tag |
| 51497 | pET28a-ClbN-NHis | NRPS module containing CATE domains with N-terminal 6His tag |