Cylindrocyclophane biosynthesis involves functionalization of an unactivated carbon center.

Nakamura H, Hamer HA, Sirasani G, Balskus EP
J Am Chem Soc. 2012 Nov 14;134(45):18518-21. doi: 10.1021/ja308318p. Epub 2012 Nov 2. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID	Plasmid	Purpose
48198	pET-28a-CylA	Expresses CylA (fatty acyl-AMP ligase) in E. coli
48199	pET-29b-CylB	Expresses CylB (acyl carrier protein) in E. coli
48200	pET-28a-CylI	Expresses CylI (type III PKS) in E. coli