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OTU Deubiquitinases Reveal Mechanisms of Linkage Specificity and Enable Ubiquitin Chain Restriction Analysis.

Mevissen TE, Hospenthal MK, Geurink PP, Elliott PR, Akutsu M, Arnaudo N, Ekkebus R, Kulathu Y, Wauer T, El Oualid F, Freund SM, Ovaa H, Komander D
Cell. 2013 Jul 3;154(1):169-84. doi: 10.1016/j.cell.2013.05.046. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
61405pOPINK-OTUD1 (full-length, aa 1-481)Expresses human OTUD1 (full-length) in E. coli.
61406pOPINK-OTUD1 (OTU+UIM, aa 287-481)Expresses human OTUD1 (OTU+UIM domains) in E. coli.
61407pOPINK-OTUD1 (OTU, aa 287-437)Expresses human OTUD1 (OTU domain) in E. coli.
61409pOPINK-OTUD2 (full-length, aa 1-348)Expresses human OTUD2 (full-length) in E. coli.
61410pOPINK-OTUD2 (OTU, aa 132-314)Expresses human OTUD2 (OTU domain) in E. coli.
61411pOPINK-OTUD3 (OTU+UBA, aa 52-275)Expresses human OTUD3 (OTU+UBA domains) in E. coli.
61412pOPINK-OTUD3 (OTU, aa 52-209)Expresses human OTUD3 (OTU domain) in E. coli.
61413pOPINK-OTUD4 (OTU, aa 22-196)Expresses human OTUD4 (OTU domain) in E. coli.
61414pOPINK-ALG13 (OTU, aa 216-353)Expresses human ALG13 (OTU domain) in E. coli.
61415pOPINK-OTUD5 (OTU, aa 171-358)Expresses human OTUD5 (OTU domain) in E. coli.
61416pOPINK-OTUD6A (full-length, aa 1-288)Expresses human OTUD6A (full-length) in E. coli.
61417pOPINK-OTUD6A (OTU, aa 128-288)Expresses human OTUD6A (OTU domain) in E. coli.
61418pOPINK-OTUD6B (full-length, aa 1-293)Expresses human OTUD6B (full-length) in E. coli.
61419pOPINK-OTUD6B (OTU, aa 133-293)Expresses human OTUD6B (OTU domain) in E. coli.
61420pOPINK-OTUB1 (full-length, aa 1-271)Expresses human OTUB1 (full-length) in E. coli.
61421pOPINK-OTUB2 (full-length, aa 1-234)Expresses human OTUB2 (full-length) in E. coli.
61581pOPINK-Cezanne (OTU, aa 53-446)Expresses human Cezanne (OTU domain) in E. coli.
61582pOPINK-Cezanne2 (OTU+UBA, aa 1-462)Expresses human Cezanne2 (OTU+UBA domains) in E. coli.
61583pOPINK-VCPIP (OTU, aa 25-561)Expresses human VCPIP (OTU domain) in E. coli.

Antibodies from Article