OTU Deubiquitinases Reveal Mechanisms of Linkage Specificity and Enable Ubiquitin Chain Restriction Analysis.

Mevissen TE, Hospenthal MK, Geurink PP, Elliott PR, Akutsu M, Arnaudo N, Ekkebus R, Kulathu Y, Wauer T, El Oualid F, Freund SM, Ovaa H, Komander D
Cell. 2013 Jul 3;154(1):169-84. doi: 10.1016/j.cell.2013.05.046. PubMed Article

Plasmids from Article

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ID	Plasmid	Purpose
61583	pOPINK-VCPIP (OTU, aa 25-561)	Expresses human VCPIP (OTU domain) in E. coli.
61582	pOPINK-Cezanne2 (OTU+UBA, aa 1-462)	Expresses human Cezanne2 (OTU+UBA domains) in E. coli.
61581	pOPINK-Cezanne (OTU, aa 53-446)	Expresses human Cezanne (OTU domain) in E. coli.
61421	pOPINK-OTUB2 (full-length, aa 1-234)	Expresses human OTUB2 (full-length) in E. coli.
61420	pOPINK-OTUB1 (full-length, aa 1-271)	Expresses human OTUB1 (full-length) in E. coli.
61419	pOPINK-OTUD6B (OTU, aa 133-293)	Expresses human OTUD6B (OTU domain) in E. coli.
61418	pOPINK-OTUD6B (full-length, aa 1-293)	Expresses human OTUD6B (full-length) in E. coli.
61417	pOPINK-OTUD6A (OTU, aa 128-288)	Expresses human OTUD6A (OTU domain) in E. coli.
61416	pOPINK-OTUD6A (full-length, aa 1-288)	Expresses human OTUD6A (full-length) in E. coli.
61415	pOPINK-OTUD5 (OTU, aa 171-358)	Expresses human OTUD5 (OTU domain) in E. coli.