RNA-guided gene activation by CRISPR-Cas9-based transcription factors.
Perez-Pinera P, Kocak DD, Vockley CM, Adler AF, Kabadi AM, Polstein LR, Thakore PI, Glass KA, Ousterout DG, Leong KW, Guilak F, Crawford GE, Reddy TE, Gersbach CA
Nat Methods. 2013 Jul 25. doi: 10.1038/nmeth.2600.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 47106 | pcDNA-dCas9 | Expresses inactivated S. pyogenes dCas9 (D10A, H840A) in mammalian cells |
| 47107 | pcDNA-dCas9-VP64 | Expresses inactivated S. pyogenes dCas9 (D10A, H840A) fused to VP64 transactivator domain in mammalian cells |
| 47108 | pSPgRNA | Expresses a S. pyogenes Cas9/dCas9 guide RNA in mammalian cells |