Lifetime imaging of a fluorescent protein sensor reveals surprising stability of ER thiol redox.
Avezov E, Cross BC, Kaminski Schierle GS, Winters M, Harding HP, Melo EP, Kaminski CF, Ron D
J Cell Biol. 2013 Apr 15;201(2):337-49. doi: 10.1083/jcb.201211155.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 47954 | ERroGFP_iE_pCDNA3 | Mammalian expression vector encoding for the ER targeted and FLAGM1 tagged redox probe roGFPiE (roGFP C147, E147b, C204) |
| 48629 | ERroGFP_iE_C204Q_pCDNA3 | Mammalian expression vector encoding for the ER targeted and FLAGM1 tagged insensitive variant of fluorescent redox probe roGFPiE with Cysteine 204 to Glutamine mutation (roGFP C147, E147b, C204Q) |
| 48630 | ERroGFP_C147S_pCDNA3 | Mammalian expression vector encoding for the ER targeted and FLAGM1 tagged insensitive variant of fluorescent redox probe roGFP with Cysteine 147 to Serine mutation (roGFP C147S, C204) |
| 48631 | roGFP_iE_C147S_pQE30 | Bacterial expression vector encoding for the 6XHis tagged insensitive variant of fluorescent redox probe roGFPiE with Cysteine 147 to Serine mutation (roGFP C147S, E147b, C204) |
| 48632 | roGFP_iE_C204S_pQE30 | Bacterial expression vector encoding for the 6XHis tagged insensitive variant of fluorescent redox probe roGFPiE with Cysteine 204 to Serine mutation (roGFP C147S, E147b, C204) |
| 48633 | roGFP_iL_pQE30 | Bacterial expression vector encoding for the 6XHis tagged redox probe roGFPiL (roGFP C147, L147b, C204) |
| 48634 | roGFP_iR_pQE30 | Bacterial expression vector encoding for the 6XHis tagged redox probe roGFPiR (roGFP C147, R147b, C204) |
| 48635 | ERrefGFP1_pCDNA3 | Mam vector encoding for the ER targeted and FLAGM1 tagged insensitive variant of fluorescent redox probe roGFPiE with mut Cys147 to Ser, del insertion147b and mut Cys204 to Glut.Referred to as ref GFP |
| 48644 | refGFP1_pQE30 | Bact exp vector encoding for the 6XHis tagged insensitive variant of the fluorescent redox probe roGFP with mut Cys147 to Serine, del insertion147b and mutated Cys204 to Glut.Referred to as refGFP |