A new method for high-resolution imaging of Ku foci to decipher mechanisms of DNA double-strand break repair.
Britton S, Coates J, Jackson SP
J Cell Biol. 2013 Jul 29.
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PubMed
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 46954 | pAAV-MCS2 | Cloning plasmid for the generation of Knock-In in human cells using rAAV. |
| 46955 | pAAV-Ku70-TC | Plasmid containing the targeting construct to insert a GFP-FLAG tag at the N-terminus of the human XRCC6 gene coding for Ku70. |
| 46956 | pEGFP-C1-FLAG | Plasmid for cloning and expression of GFP-FLAG tagged proteins (N-terminal tag). Confers resistance to G418. |
| 46957 | pEGFP-C1-FLAG-Ku70 | Plasmid for cloning and epression of GFP-FLAG tagged human Ku70 (N-terminal tag). Confers resistance to G418. |
| 46958 | pEGFP-C1-FLAG-Ku80 | Plasmid for expression of GFP-FLAG tagged human Ku80 (N-terminal tag). Confers resistance to G418. |
| 46959 | pEGFP-C1-FLAG-XRCC4 | Plasmid for expression of GFP-FLAG tagged human XRCC4 isoform 2 (N-terminal tag). Confers resistance to G418. |
| 46960 | pICE | Plasmid for cloning and constitutive or doxycycline-inducible expression of cDNAs. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression. |
| 46961 | pICE-EGFP-FLAG-Ku70siR-WT | Plasmid for constitutive or doxycycline-inducible expression of wild-type human Ku70 resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression. |
| 46962 | pICE-EGFP-FLAG-Ku70siR-Mut6E | For constitutive or doxycycline-inducible expression of mutant human Ku70 unable to bind DNA and resistant to siRNA. Confers resistance to puro. Use T-REx cells for doxycycline-inducible expression. |
| 46963 | pICE-HA-NLS-I-PpoI | For constitutive or doxycycline-inducible expression of the site specific homing nuclease I-PpoI (human codon optimized). Confers resistance to puro. Use T-REx cells for doxy-inducible expression. |