Inducible, reversible system for the rapid and complete degradation of proteins in mammalian cells.
Holland AJ, Fachinetti D, Han JS, Cleveland DW
Proc Natl Acad Sci U S A. 2012 Dec 4;109(49):E3350-7. doi: 10.1073/pnas.1216880109. Epub 2012 Nov 12.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 47328 | pBabe TIR1-9myc | For retroviral expression of E3 ubiquitin ligase osTIR1 with N-term NES and C-term 9x myc tag |
| 47329 | pcDNA5-H2B-AID-EYFP | for plasmid integration using the Flp-In system. AID at C-terminal |
| 47330 | pcDNA5-EGFP-AID-BubR1 | for plasmid integration using the Flp-In system. AID at N-terminal |
| 64945 | pBABE TIR1-9myc | For retroviral expression of E3 ubiquitin ligase osTIR1 with 9x myc tag |