Metabolic engineering of Escherichia coli for limonene and perillyl alcohol production.
Alonso-Gutierrez J, Chan R, Batth TS, Adams PD, Keasling JD, Petzold CJ, Lee TS
Metab Eng. 2013 May 29;19C:33-41. doi: 10.1016/j.ymben.2013.05.004.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 47048 | pJBEI-6409 | BglBrick plasmid (=pBbA5c-MTSAe-T1f-MBI(f)-T1002i-Ptrc-trGPPS(co)-LS) coding for MEV pathway enzymes to produce limonene from glucose in E. coli |
| 47049 | pJBEI-6410 | BglBrick plasmid (=pBbA5a-MTSAe-T1f-MBI(f)-T1002i-Ptrc-trGPPS(co)-LS) coding for MEV pathway enzymes to produce limonene from glucose in E. coli |
| 47050 | pJBEI-6411 | BglBrick plasmid (=pBbB8k-P450) coding for a cytochrome P450 and its associated reductases to oxidize limonene to perillyl alcohol in E. coli |
| 50603 | pTrc-trGPPS(CO)-LS | pTrc-trGPPS(CO)-LS: A plasmid that has truncated codon optimized geranylpyrophosphate synthase and truncated limonene synthase |
| 87950 | pBbA5c-MevT-MBI (JBEI-3085) | the overproduction of the universal building blocks to make isoprenoids (i.e. IPP and DMAPP). |