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Pseudomonas aeruginosa exotoxin Y is a promiscuous cyclase that increases endothelial tau phosphorylation and permeability.

Ochoa CD, Alexeyev M, Pastukh V, Balczon R, Stevens T
J Biol Chem. 2012 Jul 20;287(30):25407-18. doi: 10.1074/jbc.M111.301440. Epub 2012 May 25. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
46855pMA3200A lentiviral vector encoding a fusion between codon-optimized P.aeruginosa exoY and myc-tagged destabilized FKBP12
46856pMA3228A lentiviral vector encoding a fusion between codon-optimized P.aeruginosa exoY mutant (K81M) and myc-tagged destabilized FKBP12
46874pMA3379A lentiviral vector for the doxycycline-inducible expression of soluble guanylate cyclase1 alpha3
46875pMA3383A retroviral vector for the constitutive expression of soluble guanylate cyclase1 beta3
46876pMA3431A lentiviral vector for the doxycycline-inducible expression of soluble guanylate cyclase1 alpha3 mutant R592Q
46877pMA3411A retroviral vector for the constitutive expression of soluble guanylate cyclase1 beta3 double mutant (E473K and C541D)
46878pMA1662Retroviral vector, Hygromycin resistance
46879pMA3211Lentiviral vector for Tet-inducible expression. Puromycin-resistance
46880pMA3174Lentiviral vector for the dual control of expression through Tet-On promoter and C-terminal destabilizing domain

Antibodies from Article