Heritable genome editing in C. elegans via a CRISPR-Cas9 system.
Friedland AE, Tzur YB, Esvelt KM, Colaiacovo MP, Church GM, Calarco JA
Nat Methods. 2013 Jun 30. doi: 10.1038/nmeth.2532.
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PubMed
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Article
A CRISPR/Cas system designed and optimized for work in C. elegans.
The protocol for customizing the sgRNA expression plasmid can be located in the Materials and Methods section (online only) and Supplemental Material (online only) of the publication.
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 46168 | Peft-3::cas9-SV40_NLS::tbb-2 3'UTR | Codon optimized Cas9_SV40 NLS with intron |
| 46169 | PU6::unc-119_sgRNA | unc-119 targeting sgRNA |
| 46170 | PU6::klp-12_sgRNA | klp-12 targeting sgRNA |