A triple helix stabilizes the 3' ends of long noncoding RNAs that lack poly(A) tails.
Wilusz JE, JnBaptiste CK, Lu LY, Kuhn CD, Joshua-Tor L, Sharp PA
Genes Dev. 2012 Nov 1;26(21):2392-407. doi: 10.1101/gad.204438.112. Epub 2012 Oct 16.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 46834 | pCRII-TOPO CMV-cGFP-mMALAT1_3' WT Sense | Express cGFP transcript ending in the wildtype MALAT1 triple helix |
| 46835 | pCRII-TOPO CMV-cGFP-bGH Poly(A) Sense | Express cGFP transcript ending in a poly(A) tail [using the bGH poly(A) signal] |
| 46836 | pCRII-TOPO CMV-cGFP-SV40 Poly(A) Sense | Express cGFP transcript ending in a poly(A) tail [using the SV40 poly(A) signal] |
| 46837 | pCRII-TOPO CMV-cGFP-mMALAT1_3' Mut U1/U2 Sense | Express cGFP transcript ending in a mutant MALAT1 triple helix which causes the transcript to be degraded |
| 46838 | pCRII-TOPO CMV-cGFP-mMALAT1_3' Comp.14 Sense | Express cGFP transcript ending in the minimal functional MALAT1 triple helix |
| 46839 | pCRII-TOPO CMV-cGFP-mMALAT1_3' Comp.15 Sense | Express cGFP transcript ending in a mutant MALAT1 triple helix that causes the transcript to be stable but poorly translated |
| 46840 | pCRII-TOPO CMV-cGFP-mMEN b_3' Sense | Express cGFP transcript ending in the wildtype MEN beta triple helix |