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Knock-in/Knock-out (KIKO) vectors for rapid integration of large DNA sequences, including whole metabolic pathways, onto the Escherichia coli chromosome at well-characterised loci.

Sabri S, Steen JA, Bongers M, Nielsen LK, Vickers CE
Microb Cell Fact. 2013 Jun 24;12(1):60. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
46763pKIKOarsBCmE.coli genomic integration at arsB
46764pKIKOlacZCmE.coli genomic integration at lacZ
46765pKIKOrbsARCmE.coli genomic integration rbsAR locus
46766pKIKOarsBKmE.coli genomic integration at arsB
46767pKIKOlacZKmE.coli genomic integration at lacZ
46768pKIKOrbsARKmE.coli genomic integration rbsAR locus

Antibodies from Article