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Genome-wide analysis of membrane targeting by S. cerevisiae pleckstrin homology domains.

Yu JW, Mendrola JM, Audhya A, Singh S, Keleti D, DeWald DB, Murray D, Emr SD, Lemmon MA
Mol Cell. 2004 Mar 12;13(5):677-88. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
71369pGEX2TK-Num1-PHbacterial expression of PH domain for phosphoinositide binding assay
71370pGEX2TK-Osh2-PHbacterial expression of PH domain for phosphoinositide binding assay
71371pGEX2TK-Skm1-PHbacterial expression of PH domain for phosphoinositide binding assay
71372pRS426-GFP-Osh1-PHFor analysis of PH domain localization in yeast
71373pRS426-GFP-Osh2-PHFor analysis of PH domain localization in yeast
71374pRS426-GFP-Num1-PHFor analysis of PH domain localization in yeast
71375pRS426-GFP-PLCdelta-PHFor analysis of PH domain localization in yeast

Antibodies from Article