A single mutation in the core domain of the lac repressor reduces leakiness.
Gatti-Lafranconi P, Dijkman WP, Devenish SR, Hollfelder F
Microb Cell Fact. 2013 Jul 8;12:67. doi: 10.1186/1475-2859-12-67.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 46394 | pBAD-LacI | Overproduction of LacI |
| 46772 | pLIGFP_W220F | Protein overexpression with the modified LacIWF repressor |