Genome engineering of Drosophila with the CRISPR RNA-guided Cas9 nuclease.
Gratz SJ, Cummings AM, Nguyen JN, Hamm DC, Donohue LK, Harrison MM, Wildonger J, O'Connor-Giles KM
Genetics. 2013 May 24.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 45945 | pHsp70-Cas9 | The codon-optimized Cas9 nuclease under the control of the Drosophila hsp70 promoter used in Gratz, et al. (2013). Plasmid is low copy number. |
| 45946 | pU6-BbsI-chiRNA | Plasmid for expression of chiRNA under the control of the Drosophila snRNA:U6:96Ab promoter. |