Broad host range vectors for expression of proteins with (Twin-) Strep-tag, His-tag and engineered, export optimized yellow fluorescent protein.
Dammeyer T, Timmis KN, Tinnefeld P
Microb Cell Fact. 2013 May 20;12(1):49.
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PubMed
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 45936 | pTD-NStrepHis | use to create N-terminal Strep-tagII fusion proteins or dual tagged fusion with N-terminal Strep-tagII and C-terminal 6x His-tag |
| 45937 | pTD-NTwinStrep_Sm | use to create N-terminal Twin-Strep-tag fusion proteins or dual tagged fusion with N-terminal Twin-Strep-tag and C-terminal 6x His-tag |
| 45938 | pTD-NTwinStrep_Km | use to create N-terminal Twin-Strep-tag fusion proteins or dual tagged fusion with N-terminal Twin-Strep-tag and C-terminal 6x His-tag |
| 45939 | pTD-CTwinStrep | use to create C-terminal Twin-Strep-tag fusion proteins |
| 45940 | pTDpelB-NTwinStrep | use to create N-terminal PelB-Twin-Strep-tag fusion proteins or dual tagged fusion with N-terminal PelB-Twin-Strep-tag and C-terminal 6x His-tag for periplasmic translocation via PelB signal sequence |
| 45941 | pTDpelB-CTwinStrep | use to create N-terminal PelB and C-terminal Twin-Strep-tagged fusion proteins for periplasmic translocation via PelB signal sequence |
| 45942 | pTD-C_eYFPTwinStrep | expresses E.coli codon optimized EYFP-Twin-Strep fusion protein |
| 45943 | pTD-C_sfYFPTwinStrep | expresses P.putida codon optimized super-folder sfYFP-Twin-Strep fusion protein |
| 45944 | pTDpelB-C_sfYFPTwinStrep | expresses P.putida codon optimized PelB-sfYFP-Twin-Strep fusion protein for periplasmic translocation via PelB signal sequence |