Aurora B dynamics at centromeres create a diffusion-based phosphorylation gradient.
Wang E, Ballister ER, Lampson MA
J Cell Biol. 2011 Aug 22;194(4):539-49. doi: 10.1083/jcb.201103044. Epub 2011 Aug 15.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 45233 | Cenp-B DBD INCENP mCherry | Target INCENP to centromeres via CENP-B DNA binding domain (DBD) |
| 45234 | Cenp-B DBD INCENP TSS/AAA mCherry | Target INCENP (with TSS mutated to AAA) to centromeres via CENP-B DNA binding domain (DBD) |
| 45235 | Cenp-B INCENP mCherry | Target INCENP to centromeres via full length CENP-B |
| 45236 | LacI INCENP | Target INCENP to lac operator sequence |
| 45237 | Cenp-B DBD INCENP GFP | Target INCENP to centromeres via CENP-B DNA binding domain (DBD) |
| 45238 | Cenp-B INCENP GFP | Target INCENP to centromeres via full length CENP-B |