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Visualizing and manipulating focal adhesion kinase regulation in live cells.

Ritt M, Guan JL, Sivaramakrishnan S
J Biol Chem. 2013 Mar 29;288(13):8875-86. doi: 10.1074/jbc.M112.421164. Epub 2013 Feb 7. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
45047pCDNA-FRT-FAK0FAK fret sensor without helix
59119pCDNA-FRT-FAK10FAK fret sensor with 10 nm helix
59120pCDNA-FRT-FAK20FAK fret sensor with 20 nm helix
59121pCDNA-FRT-FAK30FAK fret sensor with 30 nm helix
59122pCDNA-FRT-FAK-kinase0FAK fret control sensor without FERM domain with no linker
59123pCDNA-FRT-FAK-kinase10FAK fret control sensor without FERM domain with 10 nm linker
59124pCDNA-FRT-FAK-kinase30FAK fret control sensor without FERM domain with 30 nm linker
59125pCDNA-FRT-FAK-FERM0FAK fret control sensor without kinase domain with no linker
59126pCDNA-FRT-FAK-FERM10FAK fret control sensor without kinase domain with 10 nm linker
59128pCS2-FAK0Cloning vector for FAK fret sensor without helix
59130pCS2-FAK20Cloning vector for FAK fret sensor with 20 nm helix
59131pCS2-FAK30Cloning vector for FAK fret sensor with 30 nm helix

Antibodies from Article