Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas systems.
Dicarlo JE, Norville JE, Mali P, Rios X, Aach J, Church GM.
Nucleic Acids Res
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PubMed
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Article
To function in yeast cells, we designed Cas9 protein expression constructs using constitutive and inducible promoters as well as a gRNA expression construct using the SNR52 snoRNA promoter.
A protocol for synthesizing gRNAs: hCRISPR gRNA synthesis protocol (PDF, 107 KB)
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 43802 | p414-TEF1p-Cas9-CYC1t | A human codon-optimized Cas9 for expression in S. cerevisiae (budding yeast) from the TEF1 promoter |
| 43803 | p426-SNR52p-gRNA.CAN1.Y-SUP4t | Encodes a gRNA that targets Can1.Y in yeast. |
| 43804 | p415-GalL-Cas9-CYC1t | A human codon-optimized Cas9 for expression in S. cerevisiae (budding yeast) from the GalL promoter |