Endoplasmic reticulum stress-responsive transcription factor ATF6alpha directs recruitment of the Mediator of RNA polymerase II transcription and multiple histone acetyltransferase complexes.
Sela D, Chen L, Martin-Brown S, Washburn MP, Florens L, Conaway JW, Conaway RC
J Biol Chem. 2012 Jun 29;287(27):23035-45. doi: 10.1074/jbc.M112.369504. Epub 2012 May 10.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 44150 | pGEM-HSPA5P | a fragment containing 429 bp of the human HSPA5 promoter (-282 to +147) |
| 64771 | pcDNA5-Flag-Med25 | Mammalian protein expression of flag-tagged Med25 |
| 64772 | pcDNA5-Flag-Med25-VWA | Mammalian expression of Flag-tagged Med25-VWA |
| 64773 | pET21a-Med25-VBD-6His | Bacterial expression of His-tagged Med25-VBD |
| 64774 | pET41s-GST-ATF6a-AD | bacterial expression of GST-tagged ATF6 (1-150) |