A new method to customize protein expression vectors for fast, efficient and background free parallel cloning.

Scholz J, Besir H, Strasser C, Suppmann S
BMC Biotechnol. 2013 Feb 14;13(1):12. PubMed Article

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ID	Plasmid	Purpose
125176	pCoofy65	Mammalian vector for parallel SLIC cloning containing N-terminal His6-Smt3Star (SumoStar*) and different C-tag options
122016	pCoofy64	Baculovirus vector for parallel SLIC cloning containing gp67 signal sequence, N-terminal His6-Smt3Star (SumoStar*) and different C-tag options
122015	pCoofy63	Baculovirus vector for parallel SLIC cloning containing N-terminal His6-Smt3Star (SumoStar*) and different C-tag options
122014	pCoofy62	Mammalian vector for parallel SLIC cloning containing the VEGF signal sequence for secretion, N-terminal His6-Smt3Star (SumoStar*) and different C-tag options
122013	pCoofy61	E.coli vector for parallel SLIC cloning containing N-terminal His6-eGFP with PreScission Cleavage site
122012	pCoofy60	Baculovirus vector for parallel SLIC cloning containing N-terminal His6-eGFP with PreScission Cleavage site
122011	pCoofy59	Baculovirus vector for parallel SLIC cloning containing N-terminal TrxA-His6 with PreScission Cleavage site
122009	pCoofy57	Mammalian vector for parallel SLIC cloning containing the VEGF signal sequence for secretion, N-terminal His, PreScission Cleavage site and different C-tag options
122008	pCoofy56	Mammalian vector for parallel SLIC cloning containing N-terminal TwinStrep and PreScission Cleavage site
122007	pCoofy55	Mammalian vector for parallel SLIC cloning containing N-terminal His10-SumoStar (Life Sensors) with additional PreScission Cleavage site and different C-tag options