Efficient genome editing in zebrafish using a CRISPR-Cas system.
Hwang WY, Fu Y, Reyon D, Maeder ML, Tsai SQ, Sander JD, Peterson RT, Yeh JR, Joung JK
Nat Biotechnol. 2013 Jan 29. doi: 10.1038/nbt.2501.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 42241 | Zebrafish-gRNA-0001 | |
| 42242 | Zebrafish-gRNA-0002 | |
| 42243 | Zebrafish-gRNA-0003 | |
| 42244 | Zebrafish-gRNA-0004 | |
| 42245 | Zebrafish-gRNA-0005 | |
| 42246 | Zebrafish-gRNA-0006 | |
| 42247 | Zebrafish-gRNA-0007 | |
| 42248 | Zebrafish-gRNA-0008 | |
| 42249 | Zebrafish-gRNA-0009 | |
| 42250 | DR274 | guide RNA (gRNA) expression vector used to create a gRNA to a specific sequence; uses T7 promoter for in vitro transcription |
| 42251 | MLM3613 | Expresses Cas9 nuclease (Streptococcus pyogenes) from CMV and T7 promoters |
| 42252 | MLM3639 |