A lentiviral microRNA-based system for single-copy polymerase II-regulated RNA interference in mammalian cells.

Stegmeier F, Hu G, Rickles RJ, Hannon GJ, Elledge SJ
Proc Natl Acad Sci U S A. 2005 Sep 13. 102(37):13212-7. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
11655	pPRIME-TET-GFP-recipient	3rd generation lentiviral transfer vector. pPRIME-mating recipient plasmid with a tetracycline-responsive promoter (TET) controlling GFP expression
11656	pPRIME-TET-GIN-recipient	3rd generation lentiviral transfer vector. pPRIME-mating recipient plasmid with a tetracycline-responsive promoter (TET) controlling GFP-IRES-Neo expression
11657	pPRIME-CMV-GFP-recipient	3rd generation lentiviral transfer vector. pPRIME-mating recipient plasmid with CMV promoter controlling GFP expression
11658	pPRIME-CMV-dsRed-recipient	3rd generation lentiviral transfer vector. pPRIME-mating recipient plasmid with CMV promoter controlling dsRed expression
11659	pPRIME-CMV-Neo-recipient	3rd generation lentiviral transfer vector. pPRIME-mating recipient plasmid with CMV promoter controlling Neomycin expression
11660	pPRIME-CMV-LNGFR-recipient	3rd generation lentiviral transfer vector. pPRIME-mating recipient plasmid with CMV promoter controlling low-affinity nerve growth factor receptor (LNGFR) expression
11661	pPRIME-TREX-GFP-recipient	3rd generation lentiviral transfer vector. pPRIME-mating recipient plasmid with Tet-responsive promoter (TREX) controlling GFP expression
11662	pPRIME-TET-GFP-FF3	3rd generation lentiviral transfer vector. pPRIME cloning plasmid with a tetracycline-responsive promoter (TET) controlling expression of GFP and miR30-based shRNA targeting firefly luciferase
11663	pPRIME-CMV-GFP-FF3	3rd generation lentiviral transfer vector. pPRIME cloning plasmid with CMV promoter controlling expression of GFP and miR30-based shRNA targeting firefly luciferase
11664	pPRIME-CMV-dsRed-FF3	3rd generation lentiviral transfer vector. pPRIME cloning plasmid with a CMV promoter controlling expression of dsRed and miR30-based shRNA targeting firefly luciferase
11665	pPRIME-CMV-Neo-FF3	3rd generation lentiviral transfer vector. pPRIME cloning plasmid with a CMV promoter controlling expression of Neomycin and miR30-based shRNA targeting firefly luciferase
11666	pPRIME-CMV-LNGFR-FF3	3rd generation lentiviral transfer vector. pPRIME cloning plasmid with a CMV promoter controlling expression of LNGFR and miR30-based shRNA targeting firefly luciferase
11667	pPRIME-TREX-GFP-FF3	3rd generation lentiviral transfer vector. pPRIME cloning plasmid with a Tet-responsive promoter (TREX) controlling expression of GFP and miR30-based shRNA targeting firefly luciferase
11675	BUN21/pML300 (recipient strain)
12082	DH10beta F' DOT sbcC (donor strain)