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Controlling promoter strength and regulation in Saccharomyces cerevisiae using synthetic hybrid promoters.

Blazeck J, Garg R, Reed B, Alper HS
Biotechnol Bioeng. 2012 Nov;109(11):2884-95. doi: 10.1002/bit.24552. Epub 2012 May 17. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
59992Gal4pBS2-PleumContains a promoter of a gal binding site coupled with a core promoter derived from native Leucine promoter.
59993Gal4pBS1-PleumContains a promoter of a gal binding site coupled with a core promoter derived from native Leucine promoter.
60326Gal4pBS12-PleumContains a promoter of two gal binding sites coupled with a core promoter derived from native Leucine promoter
60327Gal4pBS24-PleumContains a promoter of two gal binding sites coupled with a core promoter derived from native Leucine promoter
60328UASGAL-CU2-PleumContains a promoter comprised of derivatives from native promoters galactose, cyc and leucine.
60329Gal4pBS4-PleumContains a promoter of a gal binding site coupled with a core promoter derived from native Leucine promoter.
60330Gal4pBS3-PleumContains a promoter of a gal binding site coupled with a core promoter derived from native Leucine promoter.
60331UASGAL-CU1-PleumContains a promoter comprised of derivatives from native promoters galactose, cyc and leucine.
60332Gal4pBS13-PleumContains a promoter of two gal binding sites coupled with a core promoter derived from native Leucine promoter.
60333UASGAL-PleumContains a promoter of activating sequence derived from native galactose promoter coupled with a core promoter derived from native Leucine promoter.
60334Gal4pBS34-PleumContains a promoter of two gal binding sites coupled wiht a core promoter derived from native Leucine promoter.
60335UASGAL-PCYCContains a promoter of activating sequence derived from native galactose promoter coupled with a core promoter derived from native Leucine promoter.
60337PGALContains full length native galactose promoter
60338UASGAL-PgalContains a promoter of activating sequence derived from native galactose promoter coupled with full length native galactose promoter
60339Gal4pBS134-PleumContains a promoter of three gal binding sites derived from native galactose promoter coupled with a core promoter derived from native Leucine promoter.
60343UASgal-A9-PleumContains a promoter of activating sequence derived from native gal promoter coupled with core promoter derived from native Leucine promoter. Spacer between core and activating sequence was used.

Antibodies from Article