TimeSTAMP tagging of newly synthesized proteins.
Lin MZ, Tsien RY
Curr Protoc Protein Sci. 2010 Feb;Chapter 26:Unit 26.5. doi: 10.1002/0471140864.ps2605s59.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
42235 | pCS6-mPSD95a-TSa-HA | TimeSTAMP method for tagging and visualization of newly synthesized proteins. TimeSTAMPa cassette used for for low abundance proteins or proteins with slow ongoing synthesis rates |
42237 | pCS6-mPSD95a-TSt-HA | TimeSTAMP method for tagging and visualization of newly synthesized proteins. TimeSTAMPt cassette used for highly expressed proteins or proteins with faster ongoing synthesis rates |