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TimeSTAMP tagging of newly synthesized proteins.

Lin MZ, Tsien RY
Curr Protoc Protein Sci. 2010 Feb;Chapter 26:Unit 26.5. doi: 10.1002/0471140864.ps2605s59. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
42235pCS6-mPSD95a-TSa-HATimeSTAMP method for tagging and visualization of newly synthesized proteins. TimeSTAMPa cassette used for for low abundance proteins or proteins with slow ongoing synthesis rates
42237pCS6-mPSD95a-TSt-HATimeSTAMP method for tagging and visualization of newly synthesized proteins. TimeSTAMPt cassette used for highly expressed proteins or proteins with faster ongoing synthesis rates

Antibodies from Article