An Efficient Non-viral Method to Generate Integration-Free Human iPS Cells from Cord Blood and Peripheral Blood Cells.
Okita K, Yamakawa T, Matsumura Y, Sato Y, Amano N, Watanabe A, Goshima N, Yamanaka S
Stem Cells. 2012 Nov 29. doi: 10.1002/stem.1293.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 37624 | pCXWB-EBNA1 | Non-replicating episomal expression of EBNA1, which enhances transfection efficiency and expression from episomal plasmids |
| 37625 | pCXLE-hGLIS1 | Non-integrating (episomal) expression of human GLIS1 |
| 37626 | pCXLE-gw | Non-integrating (episomal) expression vector with Gateway cassette |
| 41813 | pCE-hOCT3/4 | Non-integrating (episomal) expression of human OCT3/4 |
| 41814 | pCE-hSK | Non-integrating (episomal) expression of human SOX2 and KLF4 |
| 41855 | pCE-hUL | Non-integrating (episomal) expression of human L-MYC and LIN28 |
| 41856 | pCE-mp53DD | Non-integrating (episomal) expression of mouse p53DD - p53 carboxy-terminal dominant-negative fragment |
| 41857 | pCXB-EBNA1 | |
| 41858 | pCE-GFP | Non-integrating (episomal) expression of EGFP |
| 41859 | pCXLE-mp53DD | Non-integrating (episomal) expression of mouse p53DD - p53 carboxy-terminal dominant-negative fragment |