Cre-lox-regulated conditional RNA interference from transgenes.

Ventura A, Meissner A, Dillon CP, McManus M, Sharp PA, Van Parijs L, Jaenisch R, Jacks T
Proc Natl Acad Sci U S A. 2004 Jul 13. 101(28):10380-5. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

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ID	Plasmid	Purpose
12166	pSicoR Dnmt1
12165	pSico Dnmt1
12093	pSico PGK GFP
12090	pSicoR p53
12089	pSico p53
12084	pSicoR PGK puro	Cre-regulated lentiviral shRNA vector. Cre addition causes both puromycin and shRNA to be recombined out of the construct, turning OFF shRNA expression.
11586	pSico PGK puro	Cre-regulated lentiviral shRNA vector. Cre addition causes the puromycin gene to be recombined out of the construct, activating shRNA expression.
11579	pSicoR	Cre-regulated lentiviral shRNA vector. Cre addition causes both EGFP and shRNA to be recombined out of the construct, turning OFF shRNA expression.
11578	pSico	Cre-regulated lentiviral shRNA vector. Cre addition causes EGFP to be recombined out of the construct, activating shRNA expression.