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Cre-lox-regulated conditional RNA interference from transgenes.

Ventura A, Meissner A, Dillon CP, McManus M, Sharp PA, Van Parijs L, Jaenisch R, Jacks T
Proc Natl Acad Sci U S A. 2004 Jul 13. 101(28):10380-5. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
11578pSicoCre-regulated lentiviral shRNA vector. Cre addition causes EGFP to be recombined out of the construct, activating shRNA expression.
11579pSicoRCre-regulated lentiviral shRNA vector. Cre addition causes both EGFP and shRNA to be recombined out of the construct, turning OFF shRNA expression.
11586pSico PGK puroCre-regulated lentiviral shRNA vector. Cre addition causes the puromycin gene to be recombined out of the construct, activating shRNA expression.
12084pSicoR PGK puroCre-regulated lentiviral shRNA vector. Cre addition causes both puromycin and shRNA to be recombined out of the construct, turning OFF shRNA expression.
12089pSico p53
12090pSicoR p53
12093pSico PGK GFP
12165pSico Dnmt1
12166pSicoR Dnmt1

Antibodies from Article