Two-step red-mediated recombination for versatile high-efficiency markerless DNA manipulation in Escherichia coli.
Tischer BK, von Einem J, Kaufer B, Osterrieder N
Biotechniques. 2006 Feb;40(2):191-7.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 41017 | pEPkan-S | PCR template for en passant (two-step Red-mediated) mutagenesis |
| 60960 | pBAD-I-SceI | Plasmid that allows an arabinose-inducible expression of the I-SceI homing endonuclease - for the en passant (two-step Red-mediated) mutagenesis |
| 60961 | pEP-EGFPin | PCR template for the insertion of eGFP by en passant (two-step Red-mediated) mutagenesis |
| 61601 | pEPkan-S2 | PCR template for en passant (two-step Red-mediated) mutagenesis |