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Target RNA-directed trimming and tailing of small silencing RNAs.

Ameres SL, Horwich MD, Hung JH, Xu J, Ghildiyal M, Weng Z, Zamore PD
Science. 2010 Jun 18;328(5985):1534-9. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
40832pAW-EGFP-2xmiR-1 (pAW-AS-21)miRNA sensor used in Drosophila cell lines
40833pAW-GFP-2xmiR-34 (pAW-AS-25)miRNA sensor used in Drosophila cell lines
40834pAW-EGFP-4xlet-7 (pAW-AS27)miRNA sensor used in Drosophila cell lines
40836pAS-1 (perf)Target RNAs used to determine complementarity requirements for in vitro tailing and degradation
40837pAS-2 (mm21)Target RNAs used to determine complementarity requirements for in vitro tailing and degradation
40838pAS-3 (mm20-21)Target RNAs used to determine complementarity requirements for in vitro tailing and degradation
40839pAS-4 (mm19-21)Target RNAs used to determine complementarity requirements for in vitro tailing and degradation
40840pAS-5 (mm18-21)Target RNAs used to determine complementarity requirements for in vitro tailing and degradation
40841pAS-6 (mm17-21)Target RNAs used to determine complementarity requirements for in vitro tailing and degradation
40842pAS-7 (mm12-21)Target RNAs used to determine complementarity requirements for in vitro tailing and degradation
40843pAS-8 (seed1-8)Target RNAs used to determine complementarity requirements for in vitro tailing and degradation
40844pAS-9 (bulge 9-11)Target RNAs used to determine complementarity requirements for in vitro tailing and degradation
40845pAS-10 (bulge 9-13)Target RNAs used to determine complementarity requirements for in vitro tailing and degradation
40846pAS-11 (bulge 9-15)Target RNAs used to determine complementarity requirements for in vitro tailing and degradation
40847pAS-12 (mm1-8)Target RNAs used to determine complementarity requirements for in vitro tailing and degradation
40848pAS-13Target RNAs used to determine complementarity requirements for in vitro tailing and degradation
40849pAS14 (mm16-21)Target RNAs used to determine complementarity requirements for in vitro tailing and degradation
40850pAS-15 (mm15-21)Target RNAs used to determine complementarity requirements for in vitro tailing and degradation
40851pAS-16 (mm14-21)Target RNAs used to determine complementarity requirements for in vitro tailing and degradation

Antibodies from Article