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Argonaute protein identity and pairing geometry determine cooperativity in mammalian RNA silencing.

Broderick JA, Salomon WE, Ryder SP, Aronin N, Zamore PD
RNA. 2011 Oct;17(10):1858-69. Epub 2011 Aug 30. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
40756mutpRL-TK 1xUTRTo evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNA
40757mutpRL-TK 2xUTRTo evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNA
40758mutpRL-TK 3xUTRTo evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNA
40759mutpRL-TK 4xUTRTo evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNA
40760mutpRL-TK 5xUTRTo evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNA
40761mutpRL-TK 6xUTRTo evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNA
40762psiCheck2 3xUTRRenilla luciferase reporter containing siRNA target sites
40763psicheck2 6xUTRRenilla luciferase reporter containing siRNA target sites
40764psiCheck2 6xmut UTRRenilla luciferase reporter containing siRNA target sites
40765mutpRL-TK 1x perfect to bulged siRNATo evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNA
40766mutpRL-TK 1x perfect to seed siRNATo evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNA
40767mutpRL-TK 1x perfect to seed plus 13-16 siRNATo evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNA

Antibodies from Article