Novel recombinant adeno-associated viruses for Cre activated and inactivated transgene expression in neurons.
Saunders A, Johnson CA, Sabatini BL
Front Neural Circuits. 2012 Jul 27;6:47. doi: 10.3389/fncir.2012.00047. eCollection 2012.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 37083 | pAAV-Ef1a-DIO-mCherry-WPRE-pA | Cre-dependent expression of mCherry |
| 37084 | pAAV-Ef1a-DIO-EGFP-WPRE-pA | Cre-dependent expression of EGFP |
| 37085 | pAAV-Ef1a-DO-EGFP-WPRE-pA | Expresses EGFP in cells lacking Cre (Cre-Off) |
| 37086 | pAAV-Ef1a-DO-ChETA-EYFP-WPRE-pA | Expresses ChETA-EYFP in cells lacking Cre (Cre-Off), for optogenetic excitation |
| 37087 | pAAV-Ef1a-DO-NpHR3.0-eYFP-WPRE-pA | Expresses NpHR3.0-eYFP in cells lacking Cre (Cre-Off), for optogenetic inhibition |
| 37088 | pAAV-Ef1a-FAS-NpHR3.0-eYFP-WPRE-pA | Expresses NpHR3.0-EYFP in cells lacking Cre (Cre-Off, flanked by alternative lox FAS sites), for optogenetic inhibition |
| 37089 | pAAV-Ef1a-FAS-ChETA-TdTomato-WPRE-pA | Expresses ChETA-tdTomato in cells lacking Cre (Cre-Off, flanked by alternative lox FAS sites), for optogenetic excitation |
| 37090 | pAAV-Ef1a-FAS-hChR2(H134R)-mCherry-WPRE-pA | Expresses humanized ChR2(H134R)-mCherry in cells lacking Cre (Cre-Off, flanked by alternative lox FAS sites), for optogenetic excitation |
| 37091 | pAAV-Ef1a-FAS-EGFP-WPRE-pA | Expresses EGFP in cells lacking Cre (Cre-Off, flanked by alternative lox FAS sites) |
| 37092 | pAAV-Ef1a-FAS-TdTomato-WPRE-pA | Expresses tdTomato in cells lacking Cre (Cre-Off, flanked by alternative lox FAS sites) |
| 37119 | pAAV-Ef1a-DO-mCherry-WPRE-pA | Expresses mCherry in cells lacking Cre (Cre-Off) |
| 37120 | pAAV-Ef1a-DO_DIO-TdTomato_EGFP-WPRE-pA | DO/DIO Cre-Switch. Expresses tdTomato in Cre negative cells, expresses EGFP in Cre positive cells |
| 37755 | pAAV-Ef1a-DIO-ChETA-TdTomato-WPRE-pA | Cre-dependent expression of ChETA-tdTomato, for optogenetic excitation |
| 37756 | pAAV-Ef1a-DO-ChETA-TdTomato-WPRE-pA | Expresses ChETA-TdTomato in cells lacking Cre (Cre-Off), for optogenetic excitation |